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mertk ko mice  (Miltenyi Biotec)


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    Miltenyi Biotec mertk ko mice
    Mertk Ko Mice, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mertk+ko+mice/Mer+Antibody%2C+anti-mouse%2C+REAfinity/pmc04162827-101-11-26
    Average 93 stars, based on 9 article reviews
    mertk ko mice - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: The Mertk Receptor Tyrosine Kinase Promotes T-B interaction Stimulated by IgD B-cell Receptor Cross-linking
    Article Snippet: .. Total T cells were isolated from the spleens of WT or Mertk-KO mice 10 days after GαmD injection using the CD4 + T cell isolation kit (Miltenyi Biotec, Auburn, CA). .. T cells were then labeled with CFSE (Life Technologies Co., Grand Island, NY) and co-cultured with B cells (stimulators) obtained from the spleens of WT or Mertk-KO mice after 4-hr priming with the same Ag (GαmD) in vivo.

    Article Title: The Mertk Receptor Tyrosine Kinase Promotes T-B interaction Stimulated by IgD B-cell Receptor Cross-linking
    Article Snippet: .. Ex vivo T cell stimulation with in vivo GamD-stimulated B cells Total T cells were isolated from the spleens of WT or Mertk-KO mice 10 days after GαmD injection using the CD4 + T cell isolation kit (Miltenyi Biotec, Auburn, CA). .. T cells were then labeled with CFSE (Life Technologies Co., Grand Island, NY) and co-cultured with B cells (stimulators) obtained from the spleens of WT or Mertk-KO mice after 4-hr priming with the same Ag (GαmD) in vivo.

    Injection:

    Article Title: The Mertk Receptor Tyrosine Kinase Promotes T-B interaction Stimulated by IgD B-cell Receptor Cross-linking
    Article Snippet: .. Total T cells were isolated from the spleens of WT or Mertk-KO mice 10 days after GαmD injection using the CD4 + T cell isolation kit (Miltenyi Biotec, Auburn, CA). .. T cells were then labeled with CFSE (Life Technologies Co., Grand Island, NY) and co-cultured with B cells (stimulators) obtained from the spleens of WT or Mertk-KO mice after 4-hr priming with the same Ag (GαmD) in vivo.

    Article Title: The Mertk Receptor Tyrosine Kinase Promotes T-B interaction Stimulated by IgD B-cell Receptor Cross-linking
    Article Snippet: .. Ex vivo T cell stimulation with in vivo GamD-stimulated B cells Total T cells were isolated from the spleens of WT or Mertk-KO mice 10 days after GαmD injection using the CD4 + T cell isolation kit (Miltenyi Biotec, Auburn, CA). .. T cells were then labeled with CFSE (Life Technologies Co., Grand Island, NY) and co-cultured with B cells (stimulators) obtained from the spleens of WT or Mertk-KO mice after 4-hr priming with the same Ag (GαmD) in vivo.

    Cell Isolation:

    Article Title: The Mertk Receptor Tyrosine Kinase Promotes T-B interaction Stimulated by IgD B-cell Receptor Cross-linking
    Article Snippet: .. Total T cells were isolated from the spleens of WT or Mertk-KO mice 10 days after GαmD injection using the CD4 + T cell isolation kit (Miltenyi Biotec, Auburn, CA). .. T cells were then labeled with CFSE (Life Technologies Co., Grand Island, NY) and co-cultured with B cells (stimulators) obtained from the spleens of WT or Mertk-KO mice after 4-hr priming with the same Ag (GαmD) in vivo.

    Article Title: The Mertk Receptor Tyrosine Kinase Promotes T-B interaction Stimulated by IgD B-cell Receptor Cross-linking
    Article Snippet: .. Ex vivo T cell stimulation with in vivo GamD-stimulated B cells Total T cells were isolated from the spleens of WT or Mertk-KO mice 10 days after GαmD injection using the CD4 + T cell isolation kit (Miltenyi Biotec, Auburn, CA). .. T cells were then labeled with CFSE (Life Technologies Co., Grand Island, NY) and co-cultured with B cells (stimulators) obtained from the spleens of WT or Mertk-KO mice after 4-hr priming with the same Ag (GαmD) in vivo.

    Ex Vivo:

    Article Title: The Mertk Receptor Tyrosine Kinase Promotes T-B interaction Stimulated by IgD B-cell Receptor Cross-linking
    Article Snippet: .. Ex vivo T cell stimulation with in vivo GamD-stimulated B cells Total T cells were isolated from the spleens of WT or Mertk-KO mice 10 days after GαmD injection using the CD4 + T cell isolation kit (Miltenyi Biotec, Auburn, CA). .. T cells were then labeled with CFSE (Life Technologies Co., Grand Island, NY) and co-cultured with B cells (stimulators) obtained from the spleens of WT or Mertk-KO mice after 4-hr priming with the same Ag (GαmD) in vivo.

    Cell Stimulation:

    Article Title: The Mertk Receptor Tyrosine Kinase Promotes T-B interaction Stimulated by IgD B-cell Receptor Cross-linking
    Article Snippet: .. Ex vivo T cell stimulation with in vivo GamD-stimulated B cells Total T cells were isolated from the spleens of WT or Mertk-KO mice 10 days after GαmD injection using the CD4 + T cell isolation kit (Miltenyi Biotec, Auburn, CA). .. T cells were then labeled with CFSE (Life Technologies Co., Grand Island, NY) and co-cultured with B cells (stimulators) obtained from the spleens of WT or Mertk-KO mice after 4-hr priming with the same Ag (GαmD) in vivo.

    In Vivo:

    Article Title: The Mertk Receptor Tyrosine Kinase Promotes T-B interaction Stimulated by IgD B-cell Receptor Cross-linking
    Article Snippet: .. Ex vivo T cell stimulation with in vivo GamD-stimulated B cells Total T cells were isolated from the spleens of WT or Mertk-KO mice 10 days after GαmD injection using the CD4 + T cell isolation kit (Miltenyi Biotec, Auburn, CA). .. T cells were then labeled with CFSE (Life Technologies Co., Grand Island, NY) and co-cultured with B cells (stimulators) obtained from the spleens of WT or Mertk-KO mice after 4-hr priming with the same Ag (GαmD) in vivo.



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    Big data analytics and human microarray reveal the key signaling pathways in atherosclerosis. Big data analytics. ( A – C ) Big data analytics for atherosclerosis with 98881cross analyses for overall signaling and 234 cross analyses in aortic arch based on up-to-date RNA-seq data from humans, mouse and rat. In IPA of Pathways and Lists, atherosclerosis was set as the keywords. Microarray in human atherosclerosis. ( D – E ) The top 50 downregulated or upregulated upstream regulators based on activation of z-score. ( F ) Graphical summary of human microarray data (orange: upregulated; blue: downregulated). QIAGEN Ingenuity Pathway Analysis (IPA: 1-atherosclerosis [carotid atherosclerotic plaque] NA CMP_2gGgljQ5SpJAn) and QIAGEN OmicSoft Land Explorer (OLE) were used to analyze microarray data in carotid atherosclerotic plaque from human patients. RNA-seq big data analytics in human . ( G ) MerTK expression in human diseases specifically in related aortic tissues (n = 427 in total), including normal control, embryo, cardiovascular disease (CVD) and respiratory tract disease (RTD)-related cardiovascular disease. MerTK expression was based on RNA-seq or scRNA-seq and was quantified by Log2 (FPKM + 0.1). Original data of RNA-seq or scRNA-seq for MerTK expression were downloaded from QIAGEN OmicSoft Land Explorer. BioGPS . ( H ) MerTK mRNA expression in human cells derived from BioGPS ( http://biogps.org ). The data were analyzed with GraphPad Prism 9.4.1 and shown as the mean ± SD.

    Journal: Redox Biology

    Article Title: Endothelial MerTK impairment accelerates the development of atherosclerosis

    doi: 10.1016/j.redox.2025.103861

    Figure Lengend Snippet: Big data analytics and human microarray reveal the key signaling pathways in atherosclerosis. Big data analytics. ( A – C ) Big data analytics for atherosclerosis with 98881cross analyses for overall signaling and 234 cross analyses in aortic arch based on up-to-date RNA-seq data from humans, mouse and rat. In IPA of Pathways and Lists, atherosclerosis was set as the keywords. Microarray in human atherosclerosis. ( D – E ) The top 50 downregulated or upregulated upstream regulators based on activation of z-score. ( F ) Graphical summary of human microarray data (orange: upregulated; blue: downregulated). QIAGEN Ingenuity Pathway Analysis (IPA: 1-atherosclerosis [carotid atherosclerotic plaque] NA CMP_2gGgljQ5SpJAn) and QIAGEN OmicSoft Land Explorer (OLE) were used to analyze microarray data in carotid atherosclerotic plaque from human patients. RNA-seq big data analytics in human . ( G ) MerTK expression in human diseases specifically in related aortic tissues (n = 427 in total), including normal control, embryo, cardiovascular disease (CVD) and respiratory tract disease (RTD)-related cardiovascular disease. MerTK expression was based on RNA-seq or scRNA-seq and was quantified by Log2 (FPKM + 0.1). Original data of RNA-seq or scRNA-seq for MerTK expression were downloaded from QIAGEN OmicSoft Land Explorer. BioGPS . ( H ) MerTK mRNA expression in human cells derived from BioGPS ( http://biogps.org ). The data were analyzed with GraphPad Prism 9.4.1 and shown as the mean ± SD.

    Article Snippet: The MerTK flox/flox mice and Tie2-Cre mice on the C57BL/6J background were generated by Cyagen US (Santa Clara, CA) and housed in the Division of Laboratory Animal Medicine at our institution with an approved ethic number of A23050.

    Techniques: Microarray, Protein-Protein interactions, RNA Sequencing, Activation Assay, Expressing, Control, Derivative Assay

    Endothelial MerTK deficiency promotes the development of atherosclerosis. ( A ) Body weight measurement in MerTK flox/flox mice and MerTK flox/flox Tie2 Cre mice of atherosclerosis model. ( B – C ) The visible formation of atherosclerotic plaque and H&E staining in aortic arch. After the mice were euthanized, the whole aortas were carefully dissected from surrounding tissue and fixed with 10 % neutral buffered formalin solution. The visible formation of atherosclerotic plaque was acquired by an AmScope Trinocular Stereo Microscope with a 12 MP digital camera. ( D ) Immunostaining for Cav-1 expression in aortic arch from MerTK flox/flox mice and MerTK flox/flox Tie2 Cre mice of atherosclerosis model. Mice were injected with a single dose of AAV8-PCSK9 particles along with a high fat diet for two months.

    Journal: Redox Biology

    Article Title: Endothelial MerTK impairment accelerates the development of atherosclerosis

    doi: 10.1016/j.redox.2025.103861

    Figure Lengend Snippet: Endothelial MerTK deficiency promotes the development of atherosclerosis. ( A ) Body weight measurement in MerTK flox/flox mice and MerTK flox/flox Tie2 Cre mice of atherosclerosis model. ( B – C ) The visible formation of atherosclerotic plaque and H&E staining in aortic arch. After the mice were euthanized, the whole aortas were carefully dissected from surrounding tissue and fixed with 10 % neutral buffered formalin solution. The visible formation of atherosclerotic plaque was acquired by an AmScope Trinocular Stereo Microscope with a 12 MP digital camera. ( D ) Immunostaining for Cav-1 expression in aortic arch from MerTK flox/flox mice and MerTK flox/flox Tie2 Cre mice of atherosclerosis model. Mice were injected with a single dose of AAV8-PCSK9 particles along with a high fat diet for two months.

    Article Snippet: The MerTK flox/flox mice and Tie2-Cre mice on the C57BL/6J background were generated by Cyagen US (Santa Clara, CA) and housed in the Division of Laboratory Animal Medicine at our institution with an approved ethic number of A23050.

    Techniques: Staining, Microscopy, Immunostaining, Expressing, Injection

    Endothelial MerTK deficiency promotes proinflammation response and activates p22 phox while inhibits ApoE expression. ( A – D ) Immunostaining for the expression of IL-1β, MCP-1, ApoE, p22 phox and NF-κB i n aortic arch from MerTK flox/flox mice and MerTK flox/flox Tie2 Cre mice that were injected with a single dose of AAV8-PCSK9 particles along with a high fat diet for two months.

    Journal: Redox Biology

    Article Title: Endothelial MerTK impairment accelerates the development of atherosclerosis

    doi: 10.1016/j.redox.2025.103861

    Figure Lengend Snippet: Endothelial MerTK deficiency promotes proinflammation response and activates p22 phox while inhibits ApoE expression. ( A – D ) Immunostaining for the expression of IL-1β, MCP-1, ApoE, p22 phox and NF-κB i n aortic arch from MerTK flox/flox mice and MerTK flox/flox Tie2 Cre mice that were injected with a single dose of AAV8-PCSK9 particles along with a high fat diet for two months.

    Article Snippet: The MerTK flox/flox mice and Tie2-Cre mice on the C57BL/6J background were generated by Cyagen US (Santa Clara, CA) and housed in the Division of Laboratory Animal Medicine at our institution with an approved ethic number of A23050.

    Techniques: Expressing, Immunostaining, Injection

    Endothelial MerTK deficiency activates pro-atherosclerotic factors. ( A – D ) Immunostaining for the expression of inflammation markers (TNF-α and IFN-γ), NADPH oxidase subunits (p47 phox and gp91 phox ), and MAPK family (ERK, p38 and JNK) in aortic arch from MerTK flox/flox mice and MerTK flox/flox Tie2 Cre mice that were injected with a single dose of AAV8-PCSK9 particles along with a high fat diet for two months.

    Journal: Redox Biology

    Article Title: Endothelial MerTK impairment accelerates the development of atherosclerosis

    doi: 10.1016/j.redox.2025.103861

    Figure Lengend Snippet: Endothelial MerTK deficiency activates pro-atherosclerotic factors. ( A – D ) Immunostaining for the expression of inflammation markers (TNF-α and IFN-γ), NADPH oxidase subunits (p47 phox and gp91 phox ), and MAPK family (ERK, p38 and JNK) in aortic arch from MerTK flox/flox mice and MerTK flox/flox Tie2 Cre mice that were injected with a single dose of AAV8-PCSK9 particles along with a high fat diet for two months.

    Article Snippet: The MerTK flox/flox mice and Tie2-Cre mice on the C57BL/6J background were generated by Cyagen US (Santa Clara, CA) and housed in the Division of Laboratory Animal Medicine at our institution with an approved ethic number of A23050.

    Techniques: Immunostaining, Expressing, Injection

    Proteomics shows the key signaling pathways in endothelial MerTK-mediated atherosclerosis . ( A ) Protein abundance in comparison of MerTK flox/flox group with MerTK flox/flox Tie2 Cre group. ( B ) Volcano plot illustrating differentially expressed proteins in the aortic arch of MerTK flox/flox Tie2 Cre vs. MerTK flox/flox . Relative protein abundance (log2) plotted against significance level (-log10 P-value), showing downregulated (blue), upregulated (red) or non-differentially expressed proteins (grey). ( C ) Graphical summary of proteomics data (orange: upregulated; blue: downregulated). ( D ) The volcano canonical pathways based on activation of z-score (lower panel). Blue: negative value. Orange: positive value. Grey: no activity pattern. Size is based on the number of genes that overlap the pathway. Big data analytics for 67,629 cross analyses for mitochondrial dysfunction based on IPA data base (upper panel). ( E – F ) The top 50 downregulated or upregulated upstream regulators based on activation of z-score.

    Journal: Redox Biology

    Article Title: Endothelial MerTK impairment accelerates the development of atherosclerosis

    doi: 10.1016/j.redox.2025.103861

    Figure Lengend Snippet: Proteomics shows the key signaling pathways in endothelial MerTK-mediated atherosclerosis . ( A ) Protein abundance in comparison of MerTK flox/flox group with MerTK flox/flox Tie2 Cre group. ( B ) Volcano plot illustrating differentially expressed proteins in the aortic arch of MerTK flox/flox Tie2 Cre vs. MerTK flox/flox . Relative protein abundance (log2) plotted against significance level (-log10 P-value), showing downregulated (blue), upregulated (red) or non-differentially expressed proteins (grey). ( C ) Graphical summary of proteomics data (orange: upregulated; blue: downregulated). ( D ) The volcano canonical pathways based on activation of z-score (lower panel). Blue: negative value. Orange: positive value. Grey: no activity pattern. Size is based on the number of genes that overlap the pathway. Big data analytics for 67,629 cross analyses for mitochondrial dysfunction based on IPA data base (upper panel). ( E – F ) The top 50 downregulated or upregulated upstream regulators based on activation of z-score.

    Article Snippet: The MerTK flox/flox mice and Tie2-Cre mice on the C57BL/6J background were generated by Cyagen US (Santa Clara, CA) and housed in the Division of Laboratory Animal Medicine at our institution with an approved ethic number of A23050.

    Techniques: Protein-Protein interactions, Quantitative Proteomics, Comparison, Activation Assay, Activity Assay

    MerTK flox/flox Tie2 Cre group demonstrates aggravated mitochondrial dysfunction compared to MerTK flox/flox group. ( A - B ) Machine learning (ML) disease pathways showing mitochondrial dysfunctions and the protein changes of mitochondrial DNA-related disorder (orange: upregulated; blue: downregulated). ( C – F ) Graphical summary for mitochondrial DNA-related disorder, mitochondrial disorder, mitochondrial cytopathy, and mitochondrial myopathy. Red: increased measurement. Green: decreased measurement. Orange: predicted activation. Bule: predicted inhibition. Glos indicates activity when the opposite of measurement. The lines indicate the predicated relationship (orange: leads to activation; blue: leads to inhibition; yellow: findings inconsistent with state of downstream molecule; grey: effect not predicted). ( G – H ) Activated or inhibited molecules associated with mitochondrial dysfunction.

    Journal: Redox Biology

    Article Title: Endothelial MerTK impairment accelerates the development of atherosclerosis

    doi: 10.1016/j.redox.2025.103861

    Figure Lengend Snippet: MerTK flox/flox Tie2 Cre group demonstrates aggravated mitochondrial dysfunction compared to MerTK flox/flox group. ( A - B ) Machine learning (ML) disease pathways showing mitochondrial dysfunctions and the protein changes of mitochondrial DNA-related disorder (orange: upregulated; blue: downregulated). ( C – F ) Graphical summary for mitochondrial DNA-related disorder, mitochondrial disorder, mitochondrial cytopathy, and mitochondrial myopathy. Red: increased measurement. Green: decreased measurement. Orange: predicted activation. Bule: predicted inhibition. Glos indicates activity when the opposite of measurement. The lines indicate the predicated relationship (orange: leads to activation; blue: leads to inhibition; yellow: findings inconsistent with state of downstream molecule; grey: effect not predicted). ( G – H ) Activated or inhibited molecules associated with mitochondrial dysfunction.

    Article Snippet: The MerTK flox/flox mice and Tie2-Cre mice on the C57BL/6J background were generated by Cyagen US (Santa Clara, CA) and housed in the Division of Laboratory Animal Medicine at our institution with an approved ethic number of A23050.

    Techniques: Activation Assay, Inhibition, Activity Assay

    Proteomics for causal network analysis shows top 50 changed regulators and microRNAs in aortic arch with MerTK flox/flox Tie2 Cre vs. MerTK flox/flox . ( A ) The top 50 activated upstream regulators and IPA prediction of MAPK family and TGFβ family networks based on activation of z-score. ( B ) The top 50 inhibited upstream regulators and IPA prediction of MAPK inhibitor and SB203580 networks based on activation of z-score. ( C ) The upregulated or downregulated microRNAs in MerTK flox/flox Tie2 Cre group compared to MerTK flox/flox group. Representative IPA prediction micorRNA-218 focusing on MAPK signaling pathway. Upregulated and downregulated proteins are highlighted in red and green, respectively, and the color depth is correlated to the fold change. Orange and blue dashed lines with arrows indicate indirect activation and inhibition, respectively. Yellow and grey dashed lines with arrows depict inconsistent effects and no prediction, respectively.

    Journal: Redox Biology

    Article Title: Endothelial MerTK impairment accelerates the development of atherosclerosis

    doi: 10.1016/j.redox.2025.103861

    Figure Lengend Snippet: Proteomics for causal network analysis shows top 50 changed regulators and microRNAs in aortic arch with MerTK flox/flox Tie2 Cre vs. MerTK flox/flox . ( A ) The top 50 activated upstream regulators and IPA prediction of MAPK family and TGFβ family networks based on activation of z-score. ( B ) The top 50 inhibited upstream regulators and IPA prediction of MAPK inhibitor and SB203580 networks based on activation of z-score. ( C ) The upregulated or downregulated microRNAs in MerTK flox/flox Tie2 Cre group compared to MerTK flox/flox group. Representative IPA prediction micorRNA-218 focusing on MAPK signaling pathway. Upregulated and downregulated proteins are highlighted in red and green, respectively, and the color depth is correlated to the fold change. Orange and blue dashed lines with arrows indicate indirect activation and inhibition, respectively. Yellow and grey dashed lines with arrows depict inconsistent effects and no prediction, respectively.

    Article Snippet: The MerTK flox/flox mice and Tie2-Cre mice on the C57BL/6J background were generated by Cyagen US (Santa Clara, CA) and housed in the Division of Laboratory Animal Medicine at our institution with an approved ethic number of A23050.

    Techniques: Activation Assay, Inhibition

    Multi-comparisons for common signaling between proteomics of MerTK flox/flox Tie2 Cre vs. MerTK flox/flox and shared atherosclerosis projects. ( A ) Upstream regulators in MerTK flox/flox Tie2 Cre vs. MerTK flox/flox compared with other atherosclerosis projects based on activation of z-score. ( B ) The volcano canonical pathways based on activation of z-score. ( C ) The toxicity functions analyses based on activation of z-score. ( D ) Diseases and biological functions analysis based on activation of z-score.

    Journal: Redox Biology

    Article Title: Endothelial MerTK impairment accelerates the development of atherosclerosis

    doi: 10.1016/j.redox.2025.103861

    Figure Lengend Snippet: Multi-comparisons for common signaling between proteomics of MerTK flox/flox Tie2 Cre vs. MerTK flox/flox and shared atherosclerosis projects. ( A ) Upstream regulators in MerTK flox/flox Tie2 Cre vs. MerTK flox/flox compared with other atherosclerosis projects based on activation of z-score. ( B ) The volcano canonical pathways based on activation of z-score. ( C ) The toxicity functions analyses based on activation of z-score. ( D ) Diseases and biological functions analysis based on activation of z-score.

    Article Snippet: The MerTK flox/flox mice and Tie2-Cre mice on the C57BL/6J background were generated by Cyagen US (Santa Clara, CA) and housed in the Division of Laboratory Animal Medicine at our institution with an approved ethic number of A23050.

    Techniques: Activation Assay

    The axis of miR-218-5p/EC MerTK /MAPK represents a novel mechanism of endothelial MerTK-mediated atherosclerosis. ( A ) Expression of p-JNK, p-p38 and p-ERK in HAECs ( B ) Immunostaining for p-ERK expression in HAECs. ( A - B ) HAECs were transfected with transfected with MerTK CRISPR/Cas9 KO Plasmid or control plasmid at 1 μg/6-well in 1 mL for 48 h. Then HAECs were incubated with apoptotic Jurkat cells at a 1:1 apoptotic cell/EC ratio. ( C ) Expression of p-JNK, p-p38 and p-ERK in HAECs. Cells were pre-transfected with miR-218–5p or miR control at 100 nM for 48 h, treated with MerTK inhibitors of UNC 569 or UNC 5293 at 500 nM for 4 h, and then were incubated with apoptotic cells at a 1:1 apoptotic cell/EC ratio for 1 h.

    Journal: Redox Biology

    Article Title: Endothelial MerTK impairment accelerates the development of atherosclerosis

    doi: 10.1016/j.redox.2025.103861

    Figure Lengend Snippet: The axis of miR-218-5p/EC MerTK /MAPK represents a novel mechanism of endothelial MerTK-mediated atherosclerosis. ( A ) Expression of p-JNK, p-p38 and p-ERK in HAECs ( B ) Immunostaining for p-ERK expression in HAECs. ( A - B ) HAECs were transfected with transfected with MerTK CRISPR/Cas9 KO Plasmid or control plasmid at 1 μg/6-well in 1 mL for 48 h. Then HAECs were incubated with apoptotic Jurkat cells at a 1:1 apoptotic cell/EC ratio. ( C ) Expression of p-JNK, p-p38 and p-ERK in HAECs. Cells were pre-transfected with miR-218–5p or miR control at 100 nM for 48 h, treated with MerTK inhibitors of UNC 569 or UNC 5293 at 500 nM for 4 h, and then were incubated with apoptotic cells at a 1:1 apoptotic cell/EC ratio for 1 h.

    Article Snippet: The MerTK flox/flox mice and Tie2-Cre mice on the C57BL/6J background were generated by Cyagen US (Santa Clara, CA) and housed in the Division of Laboratory Animal Medicine at our institution with an approved ethic number of A23050.

    Techniques: Expressing, Immunostaining, Transfection, CRISPR, Plasmid Preparation, Control, Incubation